A Shiv Sena MLA from Aurangabad district offered to resign in support of the Maratha quota demand.
Harshavardhan Jadhav, who represents Kannad assembly constituency, had recently announced to step down if the demands of the agitating Maratha community are not met.
He is the son-in-law of BJP’s state unit president Raosaheb Danve.
In his letter addressed to Legislative Assembly Speaker Haribhau Bagde, Jadhav stated that he was tendering his resignation on Wednesday afternoon.
When asked, Jadhav said he has also e-mailed his resignation to the Speaker and will personally submit it to him in Mumbai on Thursday.
The Shiv Sena, a constituent of the BJP-led state and Central governments, has 63 MLAs in the 288-member House.
The Maratha community has been demanding quota under OBC category in government jobs and education.